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The Canine Genome Project
In view of the very large spectrum of polymorphism observed within
the 300 breeds which compose the dog species, DOG appears to be a very
attractive model to identify genes involved in phenotypic, behavioural and
pathological traits.
With this in mind, we have undertook the construction of a genome
map of the Canis familiaris species. A canine Whole Genome Radiation Hybrid
panel (1) of 126 hybrid cell lines has been constructed by fusing dog
fibroblasts, irradiated at 5000 rads, with TK-hamster cells (RHDF5000).
A first canine radiation hybrid map
(Priat et al.) has been established by typing 400
markers, comprising 218 genes and 182 microsatellites, on this RH panel
The mapping of other pools of microsatellites, isolated in the lab
is in progress. The potentiel resolution of this panel has been evaluated to 500 kb. Some linkage groups have been
assigned to 10 canine chromosomes (CFA 3, 4, 9, 10, 12, 13, 18, 20, X and
Y).
Moreover, the comparison of RH with previously published genetic
groups (Lingaas et al., 1997 Mamm. Genome 8, 218; Mellersh et al., 1997
Genomics 46, 326; Werner et al., 1997 Genomics 42, 74) allowed to
successfully bridge both data on the same map. Comparison with other maps
species (human, mouse and pig) allowed to characterize regions where
synteny is conserved or disrupted.(3)
This integrated map made of type I and type II markers covers 80%
of the dog genome and will rapidly allow the localization and
characterisation of genes.
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400 canine STS and PCR conditions.
- Dog Genes
- Microsatellites
- Human ESTs
- Markers designed from conserved mammalian sequences (TOAST)
The current RH Map : Figures and Table
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Radiation Hybrid Mapping Project
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